验证数据展示
经过测试的应用
Positive WB detected in | human colon tissue |
Positive IHC detected in | human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18291-1-AP targets MEF2C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | myocyte enhancer factor 2C |
别名 | MEF2C, myocyte enhancer factor 2C |
计算分子量 | 51 kDa |
观测分子量 | 45-70 kDa |
GenBank蛋白编号 | NM_002397 |
基因名称 | MEF2C |
Gene ID (NCBI) | 4208 |
RRID | AB_1959476 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q06413 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. The immunogen used is peptide from transcription repressor of MEF2C. This antibody can recognize the transcript variant 1, transcript variant 2 and transcript variant 5 of MEF2C.
实验方案
Product Specific Protocols | |
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WB protocol for MEF2C antibody 18291-1-AP | Download protocol |
IHC protocol for MEF2C antibody 18291-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |