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验证数据展示

  • WB analysis of mouse brain using 13456-1-AP

    WB analysis of mouse brain using 13456-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13456-1-AP (MEI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 13456-1-AP

    IHC staining of human cervical cancer using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MEI1 antibody included
  • IHC staining of human cervical cancer using 13456-1-AP

    IHC staining of human cervical cancer using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MEI1 antibody included
  • IHC staining of rat testis using 13456-1-AP

    IHC staining of rat testis using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MEI1 antibody included
  • IHC staining of rat testis using 13456-1-AP

    IHC staining of rat testis using 13456-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MEI1 antibody included

MEI1 Polyclonal antibody

MEI1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13456-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MEI1, Meiosis defective protein 1, meiosis inhibitor 1, Meiosis inhibitor protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman cervical cancer tissue, human urothelial carcinoma tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MEI1 Ready-To-Use IHC Kit. MEI1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13456-1-AP targets MEI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MEI1 fusion protein Ag4253 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 meiosis inhibitor 1
别名 MEI1, Meiosis defective protein 1, meiosis inhibitor 1, Meiosis inhibitor protein 1
计算分子量 894 aa, 99 kDa
观测分子量 63 kDa
GenBank蛋白编号BC032248
基因名称 MEI1
Gene ID (NCBI) 150365
RRIDAB_2877951
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TIA1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEI1 may play a role in meiosis during spermatogenesis. It is required for normal meiotic chromosome synapsis. MEI1 may be involved in the formation of meiotic double-strand breaks (DSBs) in spermatocytes. This antibody recognizes isoform 1, 2, 3, 5, and 7 of MEI1 gene with MW 141 kDa, 67-72 kDa,49-54 kDa and 26 kDa .

实验方案

Product Specific Protocols
WB protocol for MEI1 antibody 13456-1-APDownload protocol
IHC protocol for MEI1 antibody 13456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols