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MEI4 Polyclonal antibody

MEI4 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29892-1-AP

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Synonyms

验证数据展示

  • WB analysis using 29892-1-AP

    WB analysis using 29892-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 29892-1-AP (MEI4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29892-1-AP

    IHC staining of mouse testis using 29892-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29892-1-AP (MEI4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 29892-1-AP

    IHC staining of mouse testis using 29892-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29892-1-AP (MEI4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 29892-1-AP

    IF Staining of mouse testis using 29892-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MEI4 antibody (29892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29892-1-AP targets MEI4 in WB, IHC, IF-P, ELISA applications and shows reactivity with Mouse, human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Mouse, human
Immunogen MEI4 fusion protein Ag31859 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Meiosis-specific protein MEI4
Synonyms
Calculated Molecular Weight44 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberNM_001322247.1
Gene Symbol MEI4
Gene ID (NCBI) 101928601
RRIDAB_3086182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA8MW99
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MEI4 antibody 29892-1-APDownload protocol
IHC protocol for MEI4 antibody 29892-1-APDownload protocol
IF protocol for MEI4 antibody 29892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols