MELK Polyclonal antibody
MELK Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, mouse colon tissue, mouse kidney tissue, human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy MELK Ready-To-Use IHC Kit. MELK primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11403-1-AP targets MELK in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | MELK fusion protein Ag1897 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | maternal embryonic leucine zipper kinase |
| Synonyms | hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38 |
| Calculated Molecular Weight | 651 aa, 75 kDa |
| Observed Molecular Weight | 50-70 kDa |
| GenBank Accession Number | BC014039 |
| Gene Symbol | MELK |
| Gene ID (NCBI) | 9833 |
| RRID | AB_2877762 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14680 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MELK antibody 11403-1-AP | Download protocol |
| IHC protocol for MELK antibody 11403-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Oncol MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway. | ||
Med Sci Monit Up-Regulated Maternal Embryonic Leucine Zipper Kinase Predicts Poor Prognosis of Hepatocellular Carcinoma Patients in a Chinese Han Population. | ||
Drug Des Devel Ther A Novel Tetramethylpyrazine Chalcone Hybrid- HCTMPPK, as a Potential Anti-Lung Cancer Agent by Downregulating MELK | ||
J Cancer Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma | ||
Cancer Cell Int Upregulation of MELK promotes chemoresistance and induces macrophage M2 polarization via CSF-1/JAK2/STAT3 pathway in gastric cancer |
