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验证数据展示

  • WB analysis using 82893-2-RR

    WB analysis using 82893-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82893-2-RR (MELK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82893-2-RR

    WB analysis of HeLa using 82893-2-RR

    WB result of MELK antibody (82893-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MELK transfected HeLa cells.

  • IHC staining of mouse kidney using 82893-2-RR

    IHC staining of mouse kidney using 82893-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MELK antibody included
  • IHC staining of mouse kidney using 82893-2-RR

    IHC staining of mouse kidney using 82893-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MELK antibody included
  • Affinity and Kinetic Characterization of 82893-2-RR

    Affinity and Kinetic Characterization of 82893-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-2-RR against Human MELK were performed. The affinity constant is below 1 pM.

MELK Recombinant antibody

MELK Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82893-2-RR

产品说明书

CloneNo. 230106A4

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCCIT cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MELK Ready-To-Use IHC Kit. MELK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82893-2-RR targets MELK in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 MELK fusion protein Ag1897 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 maternal embryonic leucine zipper kinase
别名 KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2
计算分子量 651 aa, 75 kDa
观测分子量50-70 kDa
GenBank蛋白编号BC014039
基因名称 MELK
Gene ID (NCBI) 9833
RRIDAB_3670619
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14680
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

实验方案

Product Specific Protocols
WB protocol for MELK antibody 82893-2-RRDownload protocol
IHC protocol for MELK antibody 82893-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols