• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MEP1A Polyclonal antibody

MEP1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24640-1-AP

Print datasheet

Synonyms

PPHA, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, Meprin A subunit alpha, Endopeptidase-2, EC:3.4.24.18

验证数据展示

  • WB analysis using 24640-1-AP

    WB analysis using 24640-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24640-1-AP (MEP1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24640-1-AP

    IHC staining of human colon cancer using 24640-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 24640-1-AP (MEP1A antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

24640-1-AP targets MEP1A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Immunogen MEP1A fusion protein Ag18950 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name meprin A, alpha (PABA peptide hydrolase)
Synonyms PPHA, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, Meprin A subunit alpha, Endopeptidase-2, EC:3.4.24.18
Calculated Molecular Weight 746 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC131714
Gene Symbol MEP1A
Gene ID (NCBI) 4224
RRIDAB_2879650
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16819
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MEP1A antibody 24640-1-APDownload protocol
IHC protocol for MEP1A antibody 24640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Comput Struct Biotechnol J

Integrated immunogenomic analysis of single-cell and bulk profiling reveals novel tumor antigens and subtype-specific therapeutic agents in lung adenocarcinoma

Authors - Saisai Tian
View Article