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验证数据展示

  • WB analysis of HepG2 using 18804-1-AP

    WB analysis of HepG2 using 18804-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18804-1-AP

    WB analysis of HeLa using 18804-1-AP

    WB result of MEPE antibody (18804-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPE transfected HeLa cells.

  • Featured Product
  • KD/KO验证

MEPE Polyclonal antibody

MEPE Polyclonal Antibody for WB, ELISA
Cat No. 18804-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

MEPE, OF45, Osteoblast/osteocyte factor 45

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A2780 cells, A375 cells, HeLa cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18804-1-AP targets MEPE in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix extracellular phosphoglycoprotein
别名 MEPE, OF45, Osteoblast/osteocyte factor 45
计算分子量 525 aa, 58 kDa
观测分子量 43 kDa, 98 kDa
GenBank蛋白编号BC128158
基因名称 MEPE
Gene ID (NCBI) 56955
RRIDAB_2143574
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ76
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a synthetic peptide (CTHGRKYHYVPHRQNNSTR) detects a major band (43 kDa) and two minor bands (98 kDa and 34 kDa).

实验方案

Product Specific Protocols
WB protocol for MEPE antibody 18804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols