验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, U-937 cells, mouse testis tissue |
| Positive IHC detected in | rat liver tissue, human lung tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27900-1-AP targets MERTK in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | human |
| Immunogen | MERTK fusion protein Ag27540 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | c-mer proto-oncogene tyrosine kinase |
| Synonyms | c mer, MER, MERTK, Proto oncogene c Mer, Receptor tyrosine kinase MerTK, RP38, Tyrosine protein kinase Mer |
| Calculated Molecular Weight | 999 aa, 110 kDa |
| Observed Molecular Weight | 160-180 kDa |
| GenBank Accession Number | BC114918 |
| Gene Symbol | MERTK |
| Gene ID (NCBI) | 10461 |
| RRID | AB_3086006 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12866 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MerTK (Mer tyrosine kinase), also known as RP38, c-Eyk, c-mer, and Tyro12, was first cloned from a human B lymphoblastoid expression library (PMID:8086340) and is one of the TAM (Tyro-3, Axl, and MerTK) receptor tyrosine kinase (RTK) family (PMID:23833304). Although this RTK, like others, can promote tumor cell proliferation to some extent, MERTK primarily lends tumor cells crucial survival advantages while promoting invasion, migration and metastasis, drug resistance, and, in the innate immune system, suppressing anti-tumor immunity (PMID:32417270). The multiple MERTK species observed are likely due to posttranslational modifications (PMID: 23585477).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MERTK antibody 27900-1-AP | Download protocol |
| IHC protocol for MERTK antibody 27900-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Med Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma | ||
Eur Arch Otorhinolaryngol Aberrant expressions of TAM receptors are associated with postoperative recurrence in chronic rhinosinusitis with nasal polyps | ||
Front Endocrinol (Lausanne) Downregulation of MerTK in circulating T cells of patients with non-proliferative diabetic retinopathy |