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验证数据展示

  • WB analysis using 67441-1-Ig

    WB analysis using 67441-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 67441-1-Ig

    IHC staining of human placenta using 67441-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 67441-1-Ig

    IHC staining of human placenta using 67441-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 67441-1-Ig

    IF Staining of HEK-293 using 67441-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MESDC2 antibody (67441-1-Ig, Clone: 1A2E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

MESDC2 Monoclonal antibody

MESDC2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67441-1-Ig

产品说明书

CloneNo. 1A2E7

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

BOCA, KIAA0081, LDLR chaperone MESD, MESD, MESDC2, Mesoderm development protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJAR cells, human placenta tissue, HeLa cells, A549 cells, Jurkat cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67441-1-Ig targets MESDC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 MESDC2 fusion protein Ag1403 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mesoderm development candidate 2
别名 BOCA, KIAA0081, LDLR chaperone MESD, MESD, MESDC2, Mesoderm development protein
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC009210
基因名称 MESDC2
Gene ID (NCBI) 23184
RRIDAB_2882677
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14696
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MESDC2 antibody 67441-1-IgDownload protocol
IHC protocol for MESDC2 antibody 67441-1-IgDownload protocol
IF protocol for MESDC2 antibody 67441-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols