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MESDC2 Monoclonal antibody

MESDC2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2E7

Cat no : 67441-1-Ig

Print datasheet

Synonyms

BOCA, KIAA0081, LDLR chaperone MESD, MESD, MESDC2, Mesoderm development protein

验证数据展示

  • WB analysis using 67441-1-Ig

    WB analysis using 67441-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 67441-1-Ig

    IHC staining of human placenta using 67441-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 67441-1-Ig

    IHC staining of human placenta using 67441-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67441-1-Ig (MESDC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 67441-1-Ig

    IF Staining of HEK-293 using 67441-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MESDC2 antibody (67441-1-Ig, Clone: 1A2E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJAR cells, human placenta tissue, HeLa cells, A549 cells, Jurkat cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67441-1-Ig targets MESDC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen MESDC2 fusion protein Ag1403 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mesoderm development candidate 2
Synonyms BOCA, KIAA0081, LDLR chaperone MESD, MESD, MESDC2, Mesoderm development protein
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC009210
Gene Symbol MESDC2
Gene ID (NCBI) 23184
RRIDAB_2882677
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14696
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MESDC2 antibody 67441-1-IgDownload protocol
IHC protocol for MESDC2 antibody 67441-1-IgDownload protocol
IF protocol for MESDC2 antibody 67441-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols