METAP2 Polyclonal antibody
METAP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, mouse pancreas tissue, mouse testis tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17040-1-AP targets METAP2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | METAP2 fusion protein Ag10564 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | methionyl aminopeptidase 2 |
| Synonyms | MAP 2, MAP2, MetAP 2, METAP2, Methionine aminopeptidase 2, methionyl aminopeptidase 2, MNPEP, p67, p67eIF2, Peptidase M 2 |
| Calculated Molecular Weight | 478 aa, 53 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC013782 |
| Gene Symbol | METAP2 |
| Gene ID (NCBI) | 10988 |
| RRID | AB_2144162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50579 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MetAP2, also known as MAP2, P67EIF2 or p67, is one of the three known methionine aminopeptidases responsible for the removal of the N-terminal translation initiator methionine from newly synthesized proteins, which is a critical step in protein maturation (PMID: 27692546, 16568094). The enzyme binds two cobalt or manganese ions in its active site (PMID: 11524009, 12718546). As higher MetAP2 expression was observed in tumor (mesothelioma, neuroblastoma, and colorectal carcinoma) cells compared with normal cells, the enzyme serves as a target for anti-angiogenic compounds of natural origin, such as fumagillin and ovalicin (PMID: 11485930, 15927358, 15102683, 9224570). Selective inhibition of MetAP2 stops vascularization and tumor growth in animal models (PMID: 12118091). MetAP2 has 3 isoforms with molecular mass of 50-53 kDa and apparent molecular mass 67 kD.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for METAP2 antibody 17040-1-AP | Download protocol |
| IF protocol for METAP2 antibody 17040-1-AP | Download protocol |
| IP protocol for METAP2 antibody 17040-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
