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METTL16 Polyclonal antibody

METTL16 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19924-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

WB, IHC, ELISA and More (1)

METT10D, METTL16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, rat testis tissue, NCI-H1299 cells, NIH/3T3 cells, mouse testis tissue
Positive IHC detected inhuman colon tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL16 Ready-To-Use IHC Kit. METTL16 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19924-1-AP targets METTL16 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, rat, mouse
文献引用反应性human, mouse, rat
免疫原 METTL16 fusion protein Ag13889 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase 10 domain containing
别名 METT10D, METTL16
计算分子量 562 aa, 64 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC001213
基因名称 METTL16
Gene ID (NCBI) 79066
RRIDAB_10639364
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86W50
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Methyltransferase-like protein 16 (METTL16) is a human RNA methyltransferase that installs m6A marks on U6 small nuclear RNA (U6 snRNA) and S-adenosylmethionine (SAM) synthetase pre-mRNA. METTL16 is considered a protective gene, suppressing the development of OC, HCC, and endocrine system tumors (PMID: 33237039, PMID: 33133142, PMID: 34187307). However, the high expression of METTL16 has been linked with a poor survival rate in patients with breast cancer (PMID: 33362863). METTL16 has 2 isoforms with molecular weights of 64 and 26 kDa.

实验方案

Product Specific Protocols
WB protocol for METTL16 antibody 19924-1-APDownload protocol
IHC protocol for METTL16 antibody 19924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.

Authors - Deguan Lv
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
humanWB

Oxid Med Cell Longev

Methylation Pattern Mediated by m6A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma.

Authors - Feng Jiang
humanWB

J Biol Chem

m6A demethylation of cytidine deaminase APOBEC3B mRNA orchestrates arsenic-induced mutagenesis.

Authors - Ming Gao
humanWB,IHC

Anal Cell Pathol (Amst)

METTL16 Inhibits the Malignant Progression of Epithelial Ovarian Cancer through the lncRNA MALAT1/β-Catenin Axis

Authors - Changshu Li
  • KD Validated
humanIHC

J Coll Physicians Surg Pak

Clinical Significance of Methyltransferase-like 16 Expression in Epithelial Ovarian Cancer

Authors - Changshu Li
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