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METTL1 Polyclonal antibody
METTL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HuH-7 cells, HeLa cells, rat brain tissue, mouse brain tissue |
Positive IP detected in | A2780 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14994-1-AP targets METTL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | METTL1 fusion protein Ag6980 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | methyltransferase like 1 |
Synonyms | miRNA, Methyltransferase-like protein 1, methyltransferase like 1, EC:2.1.1.33, EC:2.1.1.- |
Calculated Molecular Weight | 31 kDa |
Observed Molecular Weight | 31 kDa |
GenBank Accession Number | BC000550 |
Gene Symbol | METTL1 |
Gene ID (NCBI) | 4234 |
RRID | AB_2142013 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UBP6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for METTL1 antibody 14994-1-AP | Download protocol |
IHC protocol for METTL1 antibody 14994-1-AP | Download protocol |
IF protocol for METTL1 antibody 14994-1-AP | Download protocol |
IP protocol for METTL1 antibody 14994-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Dynamic methylome of internal mRNA N7-methylguanosine and its regulatory role in translation. | ||
Mol Cell N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.
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Mol Cell METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.
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Mol Cell Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.
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Nat Commun N7-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis.
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