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验证数据展示

  • WB analysis using 17184-1-AP

    WB analysis using 17184-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17184-1-AP (METTL11A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 17184-1-AP

    IHC staining of human ovary cancer using 17184-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17184-1-AP (METTL11A antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

METTL11A Polyclonal antibody

METTL11A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17184-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

methyltransferase like 11A, EC:2.1.1.244, C9orf32, Alpha N-terminal protein methyltransferase 1A, AD-003

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293T cells, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17184-1-AP targets METTL11A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 METTL11A fusion protein Ag10967 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 11A
别名 methyltransferase like 11A, EC:2.1.1.244, C9orf32, Alpha N-terminal protein methyltransferase 1A, AD-003
计算分子量 223 aa, 25 kDa
观测分子量16 kDa, 26 kDa
GenBank蛋白编号BC033234
基因名称 METTL11A
Gene ID (NCBI) 28989
RRIDAB_2142020
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL11A, also known as NTMT1 (Nα-trimethyllysine hydrolase), is an enzyme that belongs to the methyltransferase-like (METTL) protein family. It plays a crucial role in epigenetic processes, particularly in the methylation of proteins, which can influence gene expression and other cellular functions. METTL11A is regulated by other members of the METTL family, such as METTL11B and METTL13, which can either activate or inhibit its function.

实验方案

Product Specific Protocols
WB protocol for METTL11A antibody 17184-1-APDownload protocol
IHC protocol for METTL11A antibody 17184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols