• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

METTL11A Polyclonal antibody

METTL11A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17184-1-AP

Print datasheet

Synonyms

methyltransferase like 11A, EC:2.1.1.244, C9orf32, Alpha N-terminal protein methyltransferase 1A, AD-003

验证数据展示

  • WB analysis using 17184-1-AP

    WB analysis using 17184-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17184-1-AP (METTL11A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 17184-1-AP

    IHC staining of human ovary cancer using 17184-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17184-1-AP (METTL11A antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, HEK-293T cells, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17184-1-AP targets METTL11A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen METTL11A fusion protein Ag10967 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyltransferase like 11A
Synonyms methyltransferase like 11A, EC:2.1.1.244, C9orf32, Alpha N-terminal protein methyltransferase 1A, AD-003
Calculated Molecular Weight 223 aa, 25 kDa
Observed Molecular Weight16 kDa, 26 kDa
GenBank Accession NumberBC033234
Gene Symbol METTL11A
Gene ID (NCBI) 28989
RRIDAB_2142020
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV86
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL11A, also known as NTMT1 (Nα-trimethyllysine hydrolase), is an enzyme that belongs to the methyltransferase-like (METTL) protein family. It plays a crucial role in epigenetic processes, particularly in the methylation of proteins, which can influence gene expression and other cellular functions. METTL11A is regulated by other members of the METTL family, such as METTL11B and METTL13, which can either activate or inhibit its function.

实验方案

Product Specific Protocols
WB protocol for METTL11A antibody 17184-1-APDownload protocol
IHC protocol for METTL11A antibody 17184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols