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METTL14 Polyclonal antibody

METTL14 Polyclonal Antibody for WB, ELISA
Cat No. 26158-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (3)

应用

WB, ELISA and More (5)

N6-adenosine-methyltransferase non-catalytic subunit, METT L14, Methyltransferase-like protein 14, hMETTL14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NCCIT cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy METTL14 Ready-To-Use IHC Kit. METTL14 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26158-1-AP targets METTL14 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human
文献引用反应性human, pig, monkey, chicken
免疫原 METTL14 fusion protein Ag14325 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 14
别名 N6-adenosine-methyltransferase non-catalytic subunit, METT L14, Methyltransferase-like protein 14, hMETTL14
计算分子量 456 aa, 52 kDa
观测分子量55-60 kDa
GenBank蛋白编号BC007449
基因名称 METTL14
Gene ID (NCBI) 57721
RRIDAB_2800447
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HCE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.

实验方案

Product Specific Protocols
WB protocol for METTL14 antibody 26158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell Metab

Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11

Authors - Bing Zhou
humanWB

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
humanIF

Cell Res

The m6A methylome of SARS-CoV-2 in host cells.

Authors - Jun'e Liu
  • KD Validated
WB,RIP

Nat Cell Biol

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Authors - Xiang-Qian Gao
humanWB

Nat Commun

Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma

Authors - Weiwei Dai
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
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