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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 80790-1-RR (same clone as 80790-1-PBS)

    WB analysis using 80790-1-RR (same clone as 80790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80790-1-RR (METTL14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80790-1-RR (same clone as 80790-1-PBS)

    WB analysis of HeLa using 80790-1-RR (same clone as 80790-1-PBS)

    WB result of METTL14 antibody (80790-1-RR; 1:14000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL14 transfected HeLa cells. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL14 antibody included
  • IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL14 antibody included
  • IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL14 antibody included
  • Affinity and Kinetic Characterization of 80790-1-RR

    Affinity and Kinetic Characterization of 80790-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80790-1-RR against Human METTL14 were performed. The affinity constant is 9.12 nM.

  • Featured Product
  • KD/KO Validated

METTL14 Recombinant antibody, PBS Only

METTL14 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80790-1-PBS

Print datasheet

CloneNo. 2E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2E7, hMETTL14, Methyltransferase-like protein 14, METT L14, N6-adenosine-methyltransferase non-catalytic subunit

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy METTL14 Ready-To-Use IHC Kit. METTL14 primary antibody included.

产品信息

80790-1-PBS targets METTL14 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen METTL14 fusion protein Ag14325 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methyltransferase like 14
Synonyms 2E7, hMETTL14, Methyltransferase-like protein 14, METT L14, N6-adenosine-methyltransferase non-catalytic subunit
Calculated Molecular Weight 456 aa, 52 kDa
Observed Molecular Weight55-60 kDa
GenBank Accession NumberBC007449
Gene Symbol METTL14
Gene ID (NCBI) 57721
RRIDAB_2918912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HCE5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.