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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 80790-1-RR (same clone as 80790-1-PBS)

    WB analysis using 80790-1-RR (same clone as 80790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80790-1-RR (METTL14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80790-1-RR (same clone as 80790-1-PBS)

    WB analysis of HeLa using 80790-1-RR (same clone as 80790-1-PBS)

    WB result of METTL14 antibody (80790-1-RR; 1:14000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL14 transfected HeLa cells. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL14 antibody included
  • IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL14 antibody included
  • IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with METTL14 antibody included
  • Affinity and Kinetic Characterization of 80790-1-RR

    Affinity and Kinetic Characterization of 80790-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80790-1-RR against Human METTL14 were performed. The affinity constant is 9.12 nM.

  • Featured Product
  • KD/KO验证

METTL14 Recombinant antibody, PBS Only

METTL14 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80790-1-PBS

产品说明书

CloneNo. 2E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

2E7, hMETTL14, Methyltransferase-like protein 14, METT L14, N6-adenosine-methyltransferase non-catalytic subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy METTL14 Ready-To-Use IHC Kit. METTL14 primary antibody included.

产品信息

80790-1-PBS targets METTL14 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 METTL14 fusion protein Ag14325 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methyltransferase like 14
别名 2E7, hMETTL14, Methyltransferase-like protein 14, METT L14, N6-adenosine-methyltransferase non-catalytic subunit
计算分子量 456 aa, 52 kDa
观测分子量55-60 kDa
GenBank蛋白编号BC007449
基因名称 METTL14
Gene ID (NCBI) 57721
RRIDAB_2918912
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HCE5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.