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METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29314-1-AP

Print datasheet

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

验证数据展示

  • WB analysis using 29314-1-AP

    WB analysis using 29314-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29314-1-AP (METTL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29314-1-AP

    IHC staining of human lung cancer using 29314-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL3 antibody included

经过测试的应用

Positive WB detected inHeLa cells, Neuro-2a cells, K-562 cells, MCF-7 cells, COS-7 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman lung cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29314-1-AP targets METTL3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Monkey samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Monkey
Immunogen METTL3 fusion protein Ag30638 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyltransferase like 3
Synonyms IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8
Calculated Molecular Weight 64 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC001650
Gene Symbol METTL3
Gene ID (NCBI) 56339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86U44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for METTL3 antibody 29314-1-APDownload protocol
IHC protocol for METTL3 antibody 29314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols