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验证数据展示

  • WB analysis using 29314-1-AP

    WB analysis using 29314-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29314-1-AP (METTL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29314-1-AP

    IHC staining of human lung cancer using 29314-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL3 antibody included

METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29314-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat, Monkey

应用

WB, IHC, ELISA

别名

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Neuro-2a cells, K-562 cells, MCF-7 cells, COS-7 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman lung cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29314-1-AP targets METTL3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Monkey samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Monkey
免疫原 METTL3 fusion protein Ag30638 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 3
别名 IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8
计算分子量 64 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC001650
基因名称 METTL3
Gene ID (NCBI) 56339
RRIDAB_3086114
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86U44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for METTL3 antibody 29314-1-APDownload protocol
IHC protocol for METTL3 antibody 29314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols