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METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4B4A3

Cat No : 67733-2-Ig

Print datasheet

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

验证数据展示

  • WB analysis using 67733-2-Ig

    WB analysis using 67733-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67733-2-Ig (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67733-2-Ig

    WB analysis of HeLa using 67733-2-Ig

    WB result of METTL3 antibody (67733-2-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected HeLa cells.

  • IHC staining of human lung cancer using 67733-2-Ig

    IHC staining of human lung cancer using 67733-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL3 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human urothelial carcinoma tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67733-2-Ig targets METTL3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen METTL3 fusion protein Ag7110 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methyltransferase like 3
Synonyms IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC001650
Gene Symbol METTL3
Gene ID (NCBI) 56339
RRIDAB_2918503
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86U44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for METTL3 antibody 67733-2-IgDownload protocol
IHC protocol for METTL3 antibody 67733-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols