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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 68400-1-Ig (same clone as 68400-1-PBS)

    WB analysis using 68400-1-Ig (same clone as 68400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68400-1-Ig (METTL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

METTL6 Monoclonal antibody, PBS Only

METTL6 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68400-1-PBS

产品说明书

CloneNo. 1C7D4

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, ELISA

别名

methyltransferase like 6, METTL6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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68400-1-Ig

产品信息

68400-1-PBS targets METTL6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 METTL6 fusion protein Ag9714 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 methyltransferase like 6
别名 methyltransferase like 6, METTL6
计算分子量 255 aa, 30 kDa
观测分子量 30-32 kDa
GenBank蛋白编号BC022400
基因名称 METTL6
Gene ID (NCBI) 131965
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TCB7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent nontumor tissues. CRISPR/Cas9mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).