• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

METTL6 Monoclonal antibody, PBS Only

METTL6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7D4

Cat no : 68400-1-PBS

Print datasheet

Synonyms

methyltransferase like 6, METTL6

验证数据展示

  • WB analysis using 68400-1-Ig (same clone as 68400-1-PBS)

    WB analysis using 68400-1-Ig (same clone as 68400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68400-1-Ig (METTL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

68400-1-Ig

产品信息

68400-1-PBS targets METTL6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen METTL6 fusion protein Ag9714 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name methyltransferase like 6
Synonyms methyltransferase like 6, METTL6
Calculated Molecular Weight 255 aa, 30 kDa
Observed Molecular Weight 30-32 kDa
GenBank Accession NumberBC022400
Gene Symbol METTL6
Gene ID (NCBI) 131965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TCB7
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent nontumor tissues. CRISPR/Cas9mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).