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验证数据展示

  • WB analysis using 84209-4-RR (same clone as 84209-4-PBS)

    WB analysis using 84209-4-RR (same clone as 84209-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84209-4-RR (METTL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84209-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84209-4-RR

    Affinity and Kinetic Characterization of 84209-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84209-4-RR against Human METTL6 were performed. The affinity constant is below 1 pM.

METTL6 Recombinant antibody, PBS Only

METTL6 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84209-4-PBS

产品说明书

CloneNo. 241482F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

tRNA N(3)-methylcytidine methyltransferase METTL6, Methyltransferase-like protein 6, methyltransferase like 6, hMETTL6, EC:2.1.1.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84209-4-PBS targets METTL6 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 METTL6 fusion protein Ag9714 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methyltransferase like 6
别名 tRNA N(3)-methylcytidine methyltransferase METTL6, Methyltransferase-like protein 6, methyltransferase like 6, hMETTL6, EC:2.1.1.-
计算分子量 255 aa, 30 kDa
观测分子量 30-32 kDa
GenBank蛋白编号BC022400
基因名称 METTL6
Gene ID (NCBI) 131965
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TCB7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non-tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).