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METTL7A Polyclonal antibody

METTL7A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17092-1-AP

Print datasheet

Synonyms

AAM B, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348

验证数据展示

  • WB analysis using 17092-1-AP

    WB analysis using 17092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17092-1-AP (METTL7A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17092-1-AP

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL7A antibody included
  • WB analysis of HepG2 using 17092-1-AP

    WB analysis of HepG2 using 17092-1-AP

    WB result of METTL7A antibody (17092-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, mouse lung tissue, rat lung tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy METTL7A Ready-To-Use IHC Kit. METTL7A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17092-1-AP targets METTL7A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen METTL7A fusion protein Ag10013 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyltransferase like 7A
Synonyms AAM B, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC008180
Gene Symbol METTL7A
Gene ID (NCBI) 25840
RRIDAB_2878348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for METTL7A antibody 17092-1-APDownload protocol
IHC protocol for METTL7A antibody 17092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols