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  • Featured Product
  • KD/KO Validated

METTL9 Polyclonal antibody

METTL9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15120-1-AP

Print datasheet

Synonyms

EC:2.1.1.-, DREV1, DREV, CGI-81, CGI81

验证数据展示

  • WB analysis using 15120-1-AP

    WB analysis using 15120-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15120-1-AP (METTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15120-1-AP

    IHC staining of human liver cancer using 15120-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 15120-1-AP

    IHC staining of human liver cancer using 15120-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 15120-1-AP

    IF Staining of HepG2 using 15120-1-AP

    Immunofluorescent analysis of HepG2 cells using 15120-1-AP (METTL9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15120-1-AP targets METTL9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen METTL9 fusion protein Ag7191 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyltransferase like 9
Synonyms EC:2.1.1.-, DREV1, DREV, CGI-81, CGI81
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC000195
Gene Symbol METTL9
Gene ID (NCBI) 51108
RRIDAB_2878109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1A3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL9 is a broad-specificity methyltransferase that mediates the formation of the majority of 1-methylhistidine(1MH) present in mouse and human proteomes.

实验方案

Product Specific Protocols
WB protocol for METTL9 antibody 15120-1-APDownload protocol
IHC protocol for METTL9 antibody 15120-1-APDownload protocol
IF protocol for METTL9 antibody 15120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Rep

Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers.

Authors - Toshifumi Hara
  • KD Validated
View Article
humanWB,IHC

Cell Death Discov

METTL9-SLC7A11 axis promotes hepatocellular carcinoma progression through ferroptosis inhibition

Authors - Fangfang Bi
View Article