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验证数据展示

  • WB analysis using 83531-5-RR

    WB analysis using 83531-5-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 83531-5-RR (METTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83531-5-RR

    FC experiment of HepG2 using 83531-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug METTL9 Recombinant antibody (83531-5-RR, Clone:240510A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83531-5-RR

    Affinity and Kinetic Characterization of 83531-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83531-5-RR against Human METTL9 were performed. The affinity constant is below 1 pM.

METTL9 Recombinant antibody

METTL9 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83531-5-RR
CloneNo. 240510A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

DREV1, DREV, CGI-81, CGI81, CGI 81

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83531-5-RR targets METTL9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen METTL9 fusion protein Ag7191 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name methyltransferase like 9
Synonyms DREV1, DREV, CGI-81, CGI81, CGI 81
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC000195
Gene Symbol METTL9
Gene ID (NCBI) 51108
RRIDAB_3671156
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H1A3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL9 is a broad-specificity methyltransferase that mediates the formation of the majority of 1-methylhistidine(1MH) present in mouse and human proteomes.

实验方案

Product Specific Protocols
WB protocol for METTL9 antibody 83531-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols