验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, SKOV-3 cells, HCT 116 cells |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30865-1-AP targets MEX3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | MEX3A fusion protein Ag33619 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mex-3 homolog A (C. elegans) |
| 别名 | RKHD4, MEX 3A |
| 计算分子量 | 54 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | NM_001093725 |
| 基因名称 | MEX3A |
| Gene ID (NCBI) | 92312 |
| RRID | AB_3086425 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A1L020 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEX3A is a member of the MEX3 family, which widely exists in mammalian cells and encodes four different genes, MEX3A, MEX3B, MEX3C, and MEX3D. The MEX3 protein of mammals is composed of two RNA-binding KH domains and a ring domain with E3 ubiquitin ligase activity at the C terminal. The ring finger domain endows human MEX3 protein the ability to regulate ubiquitination of target proteins and then influencing their protein stability and subcellular localization.MEX3A plays complex and diverse roles in the development of various cancers. MEX3A plays a pivotal role in the post-transcriptional procession of mRNA and gene expression. MEX3A gene expression may be closely connected with malignant tumor development and progression. In most literature, MEX3A detects molecular weights between 60 and 70kDa (PMID:33159833,37845346,36354374,35490173,34976441,33159833)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEX3A antibody 30865-1-AP | Download protocol |
| IF protocol for MEX3A antibody 30865-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |