MEX3C Polyclonal antibody

MEX3C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22882-1-AP

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Synonyms

RING finger protein 194, RING finger and KH domain-containing protein 2, MEX 3C, EC:2.3.2.27, BM-013



经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22882-1-AP targets MEX3C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MEX3C fusion protein Ag18931 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mex-3 homolog C (C. elegans)
Synonyms RING finger protein 194, RING finger and KH domain-containing protein 2, MEX 3C, EC:2.3.2.27, BM-013
Calculated Molecular Weight 659 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC148855
Gene Symbol MEX3C
Gene ID (NCBI) 51320
RRIDAB_2879183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5U5Q3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEX3C(RNA-binding E3 ubiquitin-protein ligase MEX3C) is also named as RKHD2, RNF194 and may be involved in post-transcriptional regulatory mechanisms. MEX3C is necessary for normal postnatal growth and enhances the expression of local bone Igf1 expression(PMID:22927639). This protein is expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway. At least three MEX3C protein variants can be expressed by alternative splicing and alternative transcription initiation: MEX3C659AA(containing 659 amino acid residues, about 82 kDa), MEX3C464AA(about 64 kDa) and MEX3C372AA(about 50 kDa)(PMID: 28982131,27053362,22863774).

实验方案

Product Specific Protocols
WB protocol for MEX3C antibody 22882-1-APDownload protocol
IHC protocol for MEX3C antibody 22882-1-APDownload protocol
IF protocol for MEX3C antibody 22882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Gastroenterology

A High-Content Screen Identifies MicroRNAs That Regulate Liver Repopulation After Injury in Mice.

Authors - Adam M Zahm
humanWB,IF

Biol Reprod

Oocyte-Specific Expression of Mouse MEX3C652AA in the Ovary and Its Potential Role in Regulating Maternal Fos mRNA.

Authors - Xue Li