MFAP4 Polyclonal antibody

MFAP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17661-1-AP

Print datasheet

Synonyms

MFAP4



经过测试的应用

Positive WB detected inhuman kidney tissue, mouse heart tissue, mouse lung tissue, rat lung tissue
Positive IHC detected inhuman ovary tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17661-1-AP targets MFAP4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MFAP4 fusion protein Ag11920 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microfibrillar-associated protein 4
Synonyms MFAP4
Calculated Molecular Weight 255 aa, 29 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC062415
Gene Symbol MFAP4
Gene ID (NCBI) 4239
RRIDAB_2142355
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)

实验方案

Product Specific Protocols
WB protocol for MFAP4 antibody 17661-1-APDownload protocol
IHC protocol for MFAP4 antibody 17661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC

Thorax

A large lung gene expression study identifying fibulin-5 as a novel player in tissue repair in COPD.

Authors - Corry-Anke Brandsma
humanWB

J Proteome Res

Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples.

Authors - Muraoka Satoshi S
humanIHC, IF

FASEB J

The "Matrisome" reveals the characterization of skin keloid microenvironment.

Authors - Shikun Zhang
mouseWB

J Proteomics

FOXL1 + Telocytes in mouse colon orchestrate extracellular matrix biodynamics and wound repair resolution

Authors - Veronique Pomerleau