MFG-E8 Polyclonal antibody

MFG-E8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25951-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

MFGE8, Lactadherin, hP47, HMFG, EDIL1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, human placenta tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25951-1-AP targets MFG-E8 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 MFG-E8 fusion protein Ag13621 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 milk fat globule-EGF factor 8 protein
别名 MFGE8, Lactadherin, hP47, HMFG, EDIL1
计算分子量 43 kDa
观测分子量51 kDa
GenBank蛋白编号BC003610
基因名称 MFGE8
Gene ID (NCBI) 4240
RRIDAB_3669511
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ08431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), also known as Lactadherin, is a multifunctional secreted glycoprotein exhibiting versatile functions in cell physiology affecting health and diseases. It plays roles in clearing apoptotic cells, anti-inflammatory, wound healing, arterial remodeling, angiogenesis, enhancing tumorigenicity, and cancer metastasis (PMID: 23972256). MFG-E8 consists of two-repeated EGF-like domains, a mucin-like domain, and two-repeated discoidin-like domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain (PMID: 11856354). It shows ubiquitous patterns of expression in various types of cells and tissues. Altered expression of MFG-E8 causes disruptions of homeostasis and is correlated with several diseases (PMID: 27429513).

实验方案

Product Specific Protocols
WB protocol for MFG-E8 antibody 25951-1-APDownload protocol
IHC protocol for MFG-E8 antibody 25951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Heliyon

MFG-E8 promotes M2 polarization of macrophages and is associated with poor prognosis in patients with gastric cancer

Authors - Yang Li
humanWB

J Cell Mol Med

Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals

Authors - Weiming Ma
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