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MFG-E8 Polyclonal antibody

MFG-E8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25951-1-AP

Print datasheet

Synonyms

MFGE8, Lactadherin, hP47, HMFG, EDIL1

验证数据展示

  • WB analysis using 25951-1-AP

    WB analysis using 25951-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25951-1-AP (MFG-E8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 25951-1-AP

    IHC staining of human skin cancer using 25951-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25951-1-AP (MFG-E8 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, human placenta tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

25951-1-AP targets MFG-E8 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen MFG-E8 fusion protein Ag13621 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name milk fat globule-EGF factor 8 protein
Synonyms MFGE8, Lactadherin, hP47, HMFG, EDIL1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC003610
Gene Symbol MFGE8
Gene ID (NCBI) 4240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), also known as Lactadherin, is a multifunctional secreted glycoprotein exhibiting versatile functions in cell physiology affecting health and diseases. It plays roles in clearing apoptotic cells, anti-inflammatory, wound healing, arterial remodeling, angiogenesis, enhancing tumorigenicity, and cancer metastasis (PMID: 23972256). MFG-E8 consists of two-repeated EGF-like domains, a mucin-like domain, and two-repeated discoidin-like domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain (PMID: 11856354). It shows ubiquitous patterns of expression in various types of cells and tissues. Altered expression of MFG-E8 causes disruptions of homeostasis and is correlated with several diseases (PMID: 27429513).

实验方案

Product Specific Protocols
WB protocol for MFG-E8 antibody 25951-1-APDownload protocol
IHC protocol for MFG-E8 antibody 25951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Heliyon

MFG-E8 promotes M2 polarization of macrophages and is associated with poor prognosis in patients with gastric cancer

Authors - Yang Li
View Article