验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Transfected |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
10428-1-AP targets MFI2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MFI2 fusion protein Ag0700 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 |
| 别名 | MELTF, Melanotransferrin, Melanoma-associated antigen p97 |
| 计算分子量 | 80 kDa |
| 观测分子量 | 82 kDa |
| GenBank蛋白编号 | BC001875 |
| 基因名称 | MFI2 |
| Gene ID (NCBI) | 4241 |
| RRID | AB_2142592 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08582 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MFI2 (Melanotransferrin/Mtf/TRMF), also known as p97 or CD228, is an iron-binding glycoprotein belonging to the transferrin family. Alternative RNA splicing yields two forms of different length transcripts, one (the longer) bound to the membrane (mMFI2) and the other (the shorter) secreted to the peripheral environment (sMFI2) such as blood and cell supernatant. MFI2 is upregulated in tumor tissues and has been identified as a serological marker of colorectal cancer (PMID: 25216327).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MFI2 antibody 10428-1-AP | Download protocol |
| IHC protocol for MFI2 antibody 10428-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Discov Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling | ||
Reprod Sci MELTF Might Regulate Ferroptosis, Pyroptosis, and Autophagy in Platelet-Rich Plasma-Mediated Endometrial Epithelium Regeneration | ||
Front Immunol Identification of novel gene signature for lung adenocarcinoma by machine learning to predict immunotherapy and prognosis |
