• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 67487-1-Ig (same clone as 67487-1-PBS)

    WB analysis of HeLa using 67487-1-Ig (same clone as 67487-1-PBS)

    WB result of MFN2 antibody (67487-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MFN2 transfected HeLa cells. This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 67487-1-Ig (same clone as 67487-1-PBS)

    IHC staining of human heart using 67487-1-Ig (same clone as 67487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67487-1-Ig (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MFN2 antibody included
  • IHC staining of human heart using 67487-1-Ig (same clone as 67487-1-PBS)

    IHC staining of human heart using 67487-1-Ig (same clone as 67487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67487-1-Ig (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MFN2 antibody included
  • WB analysis using 67487-1-Ig (same clone as 67487-1-PBS)

    WB analysis using 67487-1-Ig (same clone as 67487-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67487-1-Ig (MFN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67487-1-Ig (same clone as 67487-1-PBS)

    IF Staining of HepG2 using 67487-1-Ig (same clone as 67487-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN2 antibody (67487-1-Ig, Clone: 5F3B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67487-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MFN2 Monoclonal antibody, PBS Only

MFN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67487-1-PBS

产品说明书

CloneNo. 5F3B3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MFN2 Ready-To-Use IHC Kit. MFN2 primary antibody included.

产品信息

67487-1-PBS targets MFN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 MFN2 fusion protein Ag29873 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mitofusin 2
别名 CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2
计算分子量 757 aa, 86 kDa
观测分子量 86 kDa
GenBank蛋白编号BC017061
基因名称 MFN2
Gene ID (NCBI) 9927
ENSEMBL Gene IDENSG00000116688
RRIDAB_2882713
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95140
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.