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MGAT2 Polyclonal antibody

MGAT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 15772-1-AP

Print datasheet

Synonyms

CDGS2, GlcNAc T II, GLCNACTII, GNT II, GNT2, MGAT2

验证数据展示

  • WB analysis of human liver using 15772-1-AP

    WB analysis of human liver using 15772-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15772-1-AP (MGAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15772-1-AP

    IHC staining of human liver using 15772-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15772-1-AP (MGAT2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 15772-1-AP

    IHC staining of human liver using 15772-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15772-1-AP (MGAT2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman liver tissue, mouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15772-1-AP targets MGAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MGAT2 fusion protein Ag8423 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Synonyms CDGS2, GlcNAc T II, GLCNACTII, GNT II, GNT2, MGAT2
Calculated Molecular Weight 447 aa, 52 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC006390
Gene Symbol MGAT2
Gene ID (NCBI) 4247
RRIDAB_2142907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10469
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MGAT2 antibody 15772-1-APDownload protocol
IHC protocol for MGAT2 antibody 15772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols