验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, Caco-2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17869-1-AP targets MGAT3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MGAT3 fusion protein Ag12352 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase |
| 别名 | GGNT3, GlcNAc T III, GNT III, GNT3, MGAT3 |
| 计算分子量 | 533 aa, 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC075026 |
| 基因名称 | MGAT3 |
| Gene ID (NCBI) | 4248 |
| RRID | AB_10597697 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q09327 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In the small intestine, ~75% of triacylglycerol (TG) synthesis occurs via the monoacylglycerol acyltransferase (MGAT) pathway.Three MGAT isoforms (MGAT1, MGAT2 and MGAT3) have been identifed based on their extensive homology to DGAT2. MGAT1 is primarily expressed in the adipose tissue, stomach and kidney but not in the small intestine. MGAT2 and MGAT3 are most highly expressed in the small intestine, suggesting that these two MGAT forms are responsible for the resynthesis of dietary TG that is packaged into chylomicrons.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MGAT3 antibody 17869-1-AP | Download protocol |
| IHC protocol for MGAT3 antibody 17869-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics MGAT3-mediated glycosylation of tetraspanin CD82 at asparagine 157 suppresses ovarian cancer metastasis by inhibiting the integrin signaling pathway.
| ||
J Biol Chem LacdiNAc synthase B4GALNT3 has a unique PA14 domain and suppresses N-glycan capping | ||
Theranostics Bisecting GlcNAc modification reverses the chemoresistance via attenuating the function of P-gp | ||
Neuropharmacology DHEC mesylate attenuates pathologies and aberrant bisecting N-glycosylation in Alzheimer's disease models | ||
Pharmacol Res Targeting aberrant glycosylation to modulate microglial response and improve cognition in models of Alzheimer's disease |
