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MGAT4A Polyclonal antibody

MGAT4A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 25109-1-AP

Print datasheet

Synonyms

GNT IVA, GNT IV, GlcNAc-T IVa, GlcNAc T IVa, EC:2.4.1.145

验证数据展示

  • IHC staining of mouse small intestine using 25109-1-AP

    IHC staining of mouse small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 25109-1-AP (MGAT4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 25109-1-AP

    IHC staining of mouse small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 25109-1-AP (MGAT4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 25109-1-AP

    WB analysis of HeLa using 25109-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25109-1-AP (MGAT4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25109-1-AP targets MGAT4A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen MGAT4A fusion protein Ag18956 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
Synonyms GNT IVA, GNT IV, GlcNAc-T IVa, GlcNAc T IVa, EC:2.4.1.145
Calculated Molecular Weight 535 aa, 62 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC127107
Gene Symbol MGAT4A
Gene ID (NCBI) 11320
RRIDAB_2879901
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MGAT4A antibody 25109-1-APDownload protocol
IHC protocol for MGAT4A antibody 25109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Biochim Biophys Acta Mol Cell Res

N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the interaction of integrin β1 with vimentin and promotes hepatocellular carcinoma cell motility

Authors - Depeng Yang
View Article