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验证数据展示

  • IHC staining of mouse small intestine using 25109-1-AP

    IHC staining of mouse small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 25109-1-AP (MGAT4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 25109-1-AP

    IHC staining of mouse small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 25109-1-AP (MGAT4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 25109-1-AP

    WB analysis of HeLa using 25109-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25109-1-AP (MGAT4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MGAT4A Polyclonal antibody

MGAT4A Polyclonal Antibody for WB, ELISA
Cat No. 25109-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, ELISA and More (1)

别名

GNT IVA, GNT IV, GlcNAc-T IVa, GlcNAc T IVa, EC:2.4.1.145

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25109-1-AP targets MGAT4A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 MGAT4A fusion protein Ag18956 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
别名 GNT IVA, GNT IV, GlcNAc-T IVa, GlcNAc T IVa, EC:2.4.1.145
计算分子量 535 aa, 62 kDa
观测分子量 68 kDa
GenBank蛋白编号BC127107
基因名称 MGAT4A
Gene ID (NCBI) 11320
RRIDAB_2879901
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM21
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MGAT4A antibody 25109-1-APDownload protocol
IHC protocol for MGAT4A antibody 25109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Biochim Biophys Acta Mol Cell Res

N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the interaction of integrin β1 with vimentin and promotes hepatocellular carcinoma cell motility

Authors - Depeng Yang
View Article