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验证数据展示

  • IHC staining of mouse liver using 30718-1-AP

    IHC staining of mouse liver using 30718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30718-1-AP

    WB analysis using 30718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 30718-1-AP

    IF Staining of U2OS using 30718-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MGAT4B antibody (30718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MGAT4B Polyclonal antibody

MGAT4B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30718-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

GnT-IVb, GNT IVB, GNT IV, GlcNAc-T IVb, EC:2.4.1.145

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in37°C incubated mouse liver tissue, human liver tissue
Positive IHC detected inmouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30718-1-AP targets MGAT4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MGAT4B fusion protein Ag33769 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
别名 GnT-IVb, GNT IVB, GNT IV, GlcNAc-T IVb, EC:2.4.1.145
计算分子量 548 aa, 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC009464
基因名称 MGAT4B
Gene ID (NCBI) 11282
RRIDAB_3086399
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ53
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MGAT4B belongs to the N-Acetylglucosaminyltransferase-IV family and catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PMID: 17006639, 10372966). MGAT4B has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor to N-glycan biosynthesis (PMID:17006639).

实验方案

Product Specific Protocols
WB protocol for MGAT4B antibody 30718-1-APDownload protocol
IHC protocol for MGAT4B antibody 30718-1-APDownload protocol
IF protocol for MGAT4B antibody 30718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols