验证数据展示

WB analysis using 14711-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14711-1-AP (MGEA5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 14711-1-AP
IP result of anti-MGEA5 (IP:14711-1-AP, 4ug; Detection:14711-1-AP 1:2000) with HepG2 cells lysate 1880 ug.
IF Staining of HepG2 using 14711-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (14711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 14711-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (14711-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
IHC staining of human pancreas using 14711-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 14711-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 40x lens).
FC experiment of HepG2 using 14711-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MGEA5 (14711-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis of HepG2 using 14711-1-AP
WB result of MGEA5 antibody (14711-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGEA5 transfected HepG2 cells.

Featured Product
KD/KO验证

MGEA5 Polyclonal antibody

MGEA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14711-1-AP

产品说明书

引用文献(82)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (4)

别名

OGA, Hexosaminidase C, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (82)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	SMMC-7721 cells, BxPC-3 cells, HepG2 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human pancreas tissue, human meningioma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 69 publications below
IHC	See 8 publications below
IF	See 12 publications below
IP	See 1 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below
RIP	See 1 publications below

产品信息

14711-1-AP targets MGEA5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, ChIP, RIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, zebrafish, drosophila
免疫原	MGEA5 fusion protein Ag6405 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	meningioma expressed antigen 5 (hyaluronidase)
别名	OGA, Hexosaminidase C, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase
计算分子量	103 kDa
观测分子量	130 kDa
GenBank蛋白编号	BC039583
基因名称	MGEA5
Gene ID (NCBI)	10724
RRID	AB_2143063
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	O60502
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).

实验方案

Product Specific Protocols
WB protocol for MGEA5 antibody 14711-1-AP	Download protocol
IHC protocol for MGEA5 antibody 14711-1-AP	Download protocol
IF protocol for MGEA5 antibody 14711-1-AP	Download protocol
IP protocol for MGEA5 antibody 14711-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins Authors - Yulin Chen View Article
human	WB	J Clin Invest Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation Authors - Di Cui View Article
human	IHC	Biomaterials PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy Authors - Lu Wang View Article
rat	WB	Redox Biol Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation. Authors - Wei Ou View Article
mouse	WB	Proc Natl Acad Sci U S A Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells. Authors - Myers Samuel A SA View Article
Drosophila	WB	Proc Natl Acad Sci U S A O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development. Authors - Pablo T Radermacher View Article

验证数据展示

WB analysis using 14711-1-AP

IP experiment of HepG2 using 14711-1-AP

IF Staining of HepG2 using 14711-1-AP

IF Staining of HepG2 using 14711-1-AP

IHC staining of human pancreas using 14711-1-AP

IHC staining of human pancreas using 14711-1-AP

FC experiment of HepG2 using 14711-1-AP

WB analysis of HepG2 using 14711-1-AP

MGEA5 Polyclonal antibody

MGEA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation

PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy

Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.

Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells.

O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development.