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MGEA5 Polyclonal antibody

MGEA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 14711-1-AP

Print datasheet

Synonyms

OGA, Hexosaminidase C, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase



经过测试的应用

Positive WB detected inSMMC-7721 cells, BxPC-3 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas tissue, human meningioma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14711-1-AP targets MGEA5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, zebrafish, drosophila
Immunogen MGEA5 fusion protein Ag6405 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name meningioma expressed antigen 5 (hyaluronidase)
Synonyms OGA, Hexosaminidase C, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC039583
Gene Symbol MGEA5
Gene ID (NCBI) 10724
RRIDAB_2143063
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60502
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).

实验方案

Product Specific Protocols
WB protocol for MGEA5 antibody 14711-1-APDownload protocol
IHC protocol for MGEA5 antibody 14711-1-APDownload protocol
IF protocol for MGEA5 antibody 14711-1-APDownload protocol
IP protocol for MGEA5 antibody 14711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen
humanWB

J Clin Invest

Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation

Authors - Di Cui
humanIHC

Biomaterials

PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy

Authors - Lu Wang
ratWB

Redox Biol

Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.

Authors - Wei Ou
mouseWB

Proc Natl Acad Sci U S A

Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells.

Authors - Myers Samuel A SA
DrosophilaWB

Proc Natl Acad Sci U S A

O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development.

Authors - Pablo T Radermacher