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验证数据展示

  • WB analysis using 14711-1-AP

    WB analysis using 14711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14711-1-AP (MGEA5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 14711-1-AP

    IP experiment of HepG2 using 14711-1-AP

    IP result of anti-MGEA5 (IP:14711-1-AP, 4ug; Detection:14711-1-AP 1:2000) with HepG2 cells lysate 1880 ug.

  • IF Staining of HepG2 using 14711-1-AP

    IF Staining of HepG2 using 14711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (14711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 14711-1-AP

    IF Staining of HepG2 using 14711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (14711-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • IHC staining of human pancreas using 14711-1-AP

    IHC staining of human pancreas using 14711-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 14711-1-AP

    IHC staining of human pancreas using 14711-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 40x lens).

  • FC experiment of HepG2 using 14711-1-AP

    FC experiment of HepG2 using 14711-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MGEA5 (14711-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HepG2 using 14711-1-AP

    WB analysis of HepG2 using 14711-1-AP

    WB result of MGEA5 antibody (14711-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGEA5 transfected HepG2 cells.

  • Featured Product
  • KD/KO Validated

MGEA5 Polyclonal antibody

MGEA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 14711-1-AP

Print datasheet

Publications(81)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (4)

Synonyms

OGA, Hexosaminidase C, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inSMMC-7721 cells, BxPC-3 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas tissue, human meningioma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14711-1-AP targets MGEA5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, zebrafish, drosophila
Immunogen MGEA5 fusion protein Ag6405 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name meningioma expressed antigen 5 (hyaluronidase)
Synonyms OGA, Hexosaminidase C, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC039583
Gene Symbol MGEA5
Gene ID (NCBI) 10724
RRIDAB_2143063
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60502
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).

实验方案

Product Specific Protocols
WB protocol for MGEA5 antibody 14711-1-APDownload protocol
IHC protocol for MGEA5 antibody 14711-1-APDownload protocol
IF protocol for MGEA5 antibody 14711-1-APDownload protocol
IP protocol for MGEA5 antibody 14711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen
View Article
humanWB

J Clin Invest

Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation

Authors - Di Cui
View Article
humanIHC

Biomaterials

PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy

Authors - Lu Wang
View Article
ratWB

Redox Biol

Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.

Authors - Wei Ou
View Article
mouseWB

Proc Natl Acad Sci U S A

Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells.

Authors - Myers Samuel A SA
View Article
DrosophilaWB

Proc Natl Acad Sci U S A

O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development.

Authors - Pablo T Radermacher
View Article