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验证数据展示

  • WB analysis using 84722-4-RR (same clone as 84722-4-PBS)

    WB analysis using 84722-4-RR (same clone as 84722-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84722-4-RR (MGEA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84722-4-PBS in a different storage buffer formulation.

  • IHC staining of human rectal cancer using 84722-4-RR (same clone as 84722-4-PBS)

    IHC staining of human rectal cancer using 84722-4-RR (same clone as 84722-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84722-4-RR (MGEA5 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84722-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84722-4-RR (same clone as 84722-4-PBS)

    IF Staining of HepG2 using 84722-4-RR (same clone as 84722-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (84722-4-RR, Clone: 242263A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84722-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84722-4-RR

    Affinity and Kinetic Characterization of 84722-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84722-4-RR against Human MGEA5 were performed. The affinity constant is below 1 pM.

MGEA5 Recombinant antibody, PBS Only

MGEA5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84722-4-PBS

产品说明书

CloneNo. 242263A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

OGA, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase, Beta-N-acetylglucosaminidase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84722-4-PBS targets MGEA5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 MGEA5 fusion protein Ag6405 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 meningioma expressed antigen 5 (hyaluronidase)
别名 OGA, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase, Beta-N-acetylglucosaminidase
计算分子量 103 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC039583
基因名称 MGEA5
Gene ID (NCBI) 10724
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60502
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).