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MGLL Polyclonal antibody

MGLL Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14986-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

HU K5, HUK5, Lysophospholipase homolog, Lysophospholipase like, MGL, MGLL, Monoglyceride lipase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, SMMC-7721 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MGLL Ready-To-Use IHC Kit. MGLL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14986-1-AP targets MGLL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 MGLL fusion protein Ag6970 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 monoglyceride lipase
别名 HU K5, HUK5, Lysophospholipase homolog, Lysophospholipase like, MGL, MGLL, Monoglyceride lipase
计算分子量 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC000551
基因名称 MGLL
Gene ID (NCBI) 11343
RRIDAB_2143189
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99685
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).

实验方案

Product Specific Protocols
WB protocol for MGLL antibody 14986-1-APDownload protocol
IP protocol for MGLL antibody 14986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Elife

p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.

Authors - Shuting Xia
humanWB

J Ethnopharmacol

Qihuang Zhuyu Formula Modulates Mitochondrial Function to Inhibit Platelet Activation via Endocannabinoid Signaling Mediated by the SIRT1/PPARα Pathway

Authors - Ruijie Shi
humanIHC

Front Oncol

Monoacylglycerol Lipase Knockdown Inhibits Cell Proliferation and Metastasis in Lung Adenocarcinoma.

Authors - Hao Zhang
  • KD Validated
ratWB

J Physiol Biochem

Sleep deprivation induced fat accumulation in the visceral white adipose tissue by suppressing SIRT1/FOXO1/ATGL pathway activation

Authors - Wei Wang
mouseWB

Neuroscience

The Endocannabinoid System Contributes to Memory Deficits Induced by Rapid-eye-movement Sleep Deprivation in Adolescent Mice.

Authors - Jinming Zhang
human,ratWB,IHC

Cell Death Discov

Intermittent hypoxia-induced METTL3 downregulation facilitates MGLL-mediated lipolysis of adipocytes in OSAS

Authors - Xiuji Huang
  • KD Validated
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