验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, A549 cells, HeLa cells, human platelets tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67846-1-Ig targets MGLL in WB, ELISA applications and shows reactivity with Human, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, pig |
| 免疫原 | MGLL fusion protein Ag7052 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | monoglyceride lipase |
| 别名 | HU K5, HUK5, Lysophospholipase homolog, Lysophospholipase like, MGL, MGLL, monoglyceride lipase |
| 计算分子量 | 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC000551 |
| 基因名称 | MGLL |
| Gene ID (NCBI) | 11343 |
| RRID | AB_2918608 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99685 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MGLL antibody 67846-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |