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MGME1 Monoclonal antibody

MGME1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C4G1

Cat no : 67468-1-Ig

Print datasheet

Synonyms

bA504H3.4, C20orf72, FLJ14597, MGME1

验证数据展示

  • IHC staining of human lung cancer using 67468-1-Ig

    IHC staining of human lung cancer using 67468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 67468-1-Ig

    IHC staining of human lung cancer using 67468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 67468-1-Ig

    WB analysis using 67468-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67468-1-Ig

    WB analysis of HeLa using 67468-1-Ig

    WB result of MGME1 antibody (67468-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGME1 transfected HeLa cells.

经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, HepG2 cells, LNCaP cells, A549 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67468-1-Ig targets MGME1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen MGME1 fusion protein Ag19644 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chromosome 20 open reading frame 72
Synonyms bA504H3.4, C20orf72, FLJ14597, MGME1
Calculated Molecular Weight 344 aa, 39 kDa
Observed Molecular Weight 36-39 kDa
GenBank Accession NumberBC016869
Gene Symbol MGME1
Gene ID (NCBI) 92667
RRIDAB_2882697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BQP7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MGME1 antibody 67468-1-IgDownload protocol
IHC protocol for MGME1 antibody 67468-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

MGME1 associates with poor prognosis and is vital for cell proliferation in lower-grade glioma

Authors - Feng Xiao
View Article