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  • Featured Product
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MGMT Monoclonal antibody, PBS Only

MGMT Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H2C9

Cat No : 67476-1-PBS

Print datasheet

Synonyms

Homo sapiens (Human), MGMT

验证数据展示

  • WB analysis using 67476-1-Ig (same clone as 67476-1-PBS)

    WB analysis using 67476-1-Ig (same clone as 67476-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67476-1-Ig (MGMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67476-1-Ig (same clone as 67476-1-PBS)

    IF Staining of HepG2 using 67476-1-Ig (same clone as 67476-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGMT antibody (67476-1-Ig, Clone: 1H2C9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67476-1-Ig (same clone as 67476-1-PBS)

    WB analysis of HeLa using 67476-1-Ig (same clone as 67476-1-PBS)

    WB result of MGMT antibody (67476-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGMT transfected HeLa cells. This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MGMT Ready-To-Use IHC Kit. MGMT primary antibody included.

产品信息

67476-1-PBS targets MGMT in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen MGMT fusion protein Ag29936 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name O-6-methylguanine-DNA methyltransferase
Synonyms Homo sapiens (Human), MGMT
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC000824
Gene Symbol MGMT
Gene ID (NCBI) 4255
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16455
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]