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验证数据展示

  • WB analysis using 67476-1-Ig (same clone as 67476-1-PBS)

    WB analysis using 67476-1-Ig (same clone as 67476-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67476-1-Ig (MGMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67476-1-Ig (same clone as 67476-1-PBS)

    IF Staining of HepG2 using 67476-1-Ig (same clone as 67476-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGMT antibody (67476-1-Ig, Clone: 1H2C9 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 67476-1-Ig (same clone as 67476-1-PBS)

    FC experiment of Jurkat using 67476-1-Ig (same clone as 67476-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MGMT (67476-1-Ig, Clone:1H2C9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67476-1-Ig (same clone as 67476-1-PBS)

    WB analysis of HeLa using 67476-1-Ig (same clone as 67476-1-PBS)

    WB result of MGMT antibody (67476-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGMT transfected HeLa cells. This data was developed using the same antibody clone with 67476-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MGMT Monoclonal antibody, PBS Only

MGMT Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67476-1-PBS

产品说明书

CloneNo. 1H2C9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

O-6-methylguanine-DNA-alkyltransferase, Methylated-DNA--protein-cysteine methyltransferase, EC:2.1.1.63, 6-O-methylguanine-DNA methyltransferase, 1H2C9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MGMT Ready-To-Use IHC Kit. MGMT primary antibody included.

产品信息

67476-1-PBS targets MGMT in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 MGMT fusion protein Ag29936 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 O-6-methylguanine-DNA methyltransferase
别名 O-6-methylguanine-DNA-alkyltransferase, Methylated-DNA--protein-cysteine methyltransferase, EC:2.1.1.63, 6-O-methylguanine-DNA methyltransferase, 1H2C9
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC000824
基因名称 MGMT
Gene ID (NCBI) 4255
RRIDAB_2882703
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16455
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]