验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11285-1-AP targets RNF156 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RNF156 fusion protein Ag1808 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mahogunin, ring finger 1 |
| 别名 | KIAA0544, mahogunin, ring finger 1, MGRN1, RING finger protein 156, RNF156 |
| 计算分子量 | 63 kDa |
| 观测分子量 | 58-64 kDa |
| GenBank蛋白编号 | BC014199 |
| 基因名称 | MGRN1 |
| Gene ID (NCBI) | 23295 |
| RRID | AB_2143351 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60291 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MGRN1(E3 ubiquitin-protein ligase) mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. It also plays a role in the regulation of endosome-to-lysosome trafficking. Human and mouse mahogunin exhibit a similar expression pattern across multiple tissues with particularly high levels in brain(PMID: 12560552). It has 4 isoforms produced by alternative splicing with the molecular mass of 58-64 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF156 antibody 11285-1-AP | Download protocol |
| IHC protocol for RNF156 antibody 11285-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival.
| ||
Dev Cell A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development. | ||
Pigment Cell Melanoma Res MGRN1-dependent pigment-type switching requires its ubiquitination activity but not its interaction with TSG101 or NEDD4. | ||
Development Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength. | ||
Front Oncol Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients. | ||
J Biol Chem Mahoganoid and mahogany mutations rectify the obesity of the yellow mouse by effects on endosomal traffic of MC4R protein.
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