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验证数据展示

  • WB analysis using 15906-1-AP

    WB analysis using 15906-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15906-1-AP (MGST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15906-1-AP

    IHC staining of human liver using 15906-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15906-1-AP (MGST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 15906-1-AP

    IHC staining of human liver using 15906-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15906-1-AP (MGST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 15906-1-AP

    IF Staining of MCF-7 using 15906-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MGST1 antibody (15906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MGST1 Polyclonal antibody

MGST1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15906-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Microsomal GST-I, Microsomal GST-1, Microsomal glutathione S-transferase 1, MGST, GST12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse lung tissue，rat liver tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15906-1-AP targets MGST1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MGST1 fusion protein Ag8498 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microsomal glutathione S-transferase 1
别名 Microsomal GST-I, Microsomal GST-1, Microsomal glutathione S-transferase 1, MGST, GST12
计算分子量 155 aa, 18 kDa
观测分子量51 kDa
GenBank蛋白编号BC005923
基因名称 MGST1
Gene ID (NCBI) 4257
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10620
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Microsomal glutathione S-transferase 1 (MGST1) is a membrane-bound transferase involved in exogenous detoxification and protection of cells from oxidative stress. MGST1 is mainly localized in the mitochondria, the plasma membrane of the endoplasmic reticulum (ER). The active form of MGST1 is a noncovalently associated homotrimer that binds one substrate glutathione molecule/trimer (PMID: 14726533; PMID: 11101503). The theoretical molecular weight of MGST1 is 17 kDa, and its homomeric molecular weight is 51-53 kDa.

实验方案

Product Specific Protocols
WB protocol for MGST1 antibody 15906-1-APDownload protocol
IHC protocol for MGST1 antibody 15906-1-APDownload protocol
IF protocol for MGST1 antibody 15906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols