验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17481-1-AP targets MIA3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, zebrafish |
| 免疫原 | MIA3 fusion protein Ag11514 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | melanoma inhibitory activity family, member 3 |
| 别名 | ARNT, C219 reactive peptide, D320, KIAA0268, MIA3, TANGO, TANGO1, UNQ6077 |
| 计算分子量 | 214 kDa |
| 观测分子量 | 300 kDa |
| GenBank蛋白编号 | BC047116 |
| 基因名称 | MIA3 |
| Gene ID (NCBI) | 375056 |
| RRID | AB_2878411 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5JRA6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIA3 antibody 17481-1-AP | Download protocol |
| IHC protocol for MIA3 antibody 17481-1-AP | Download protocol |
| IP protocol for MIA3 antibody 17481-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Endocrinol (Lausanne) MIA SH3 Domain ER Export Factor 3 Deficiency Prevents Neointimal Formation by Restoring BAT-Like PVAT and Decreasing VSMC Proliferation and Migration.
| ||
Arthritis Rheumatol Caspase-1 Enhances TANGO1 Expression to Promote Procollagen Export from the Endoplasmic Reticulum in Systemic Sclerosis Contributing to Fibrosis
| ||
Elife Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset | ||
Mol Cell Biochem MIA3 promotes the degradation of GSH (glutathione) by binding to CHAC1, thereby promoting the progression of hepatocellular carcinoma
| ||
Dev Cell Endoplasmic reticulum exit sites are segregated for secretion based on cargo size |
