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MIB1 Recombinant antibody

MIB1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240676E4

Cat no : 83718-5-RR

Print datasheet

Synonyms

DIP-1, DIP1, DIP 1, DAPK-interacting protein 1, DAPK interacting protein 1

验证数据展示

  • WB analysis using 83718-5-RR

    WB analysis using 83718-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83718-5-RR (MIB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83718-5-RR

    FC experiment of HeLa using 83718-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of SH-SY5Y using 83718-5-RR

    FC experiment of SH-SY5Y using 83718-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83718-5-RR

    Affinity and Kinetic Characterization of 83718-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83718-5-RR against Human MIB1 were performed. The affinity constant is 5.15 nM.

经过测试的应用

Positive WB detected inHeLa cells, DU 145 cells, HEK-293 cells, K-562 cells, C6 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells, SH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83718-5-RR is MIB1 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MIB1 fusion protein Ag2507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mindbomb homolog 1 (Drosophila)
Synonyms DIP-1, DIP1, DIP 1, DAPK-interacting protein 1, DAPK interacting protein 1
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC022403
Gene Symbol MIB1
Gene ID (NCBI) 57534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86YT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053).

实验方案

Product Specific Protocols
WB protocol for MIB1 antibody 83718-5-RRDownload protocol
FC protocol for MIB1 antibody 83718-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols