验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, DU 145 cells, HEK-293 cells, K-562 cells, C6 cells, mouse brain tissue, rat brain tissue |
Positive FC (Intra) detected in | HeLa cells, SH-SY5Y cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83718-5-RR targets MIB1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | MIB1 fusion protein Ag2507 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | mindbomb homolog 1 (Drosophila) |
Synonyms | DIP-1, DIP1, DIP 1, DAPK-interacting protein 1, DAPK interacting protein 1 |
Calculated Molecular Weight | 110 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | BC022403 |
Gene Symbol | MIB1 |
Gene ID (NCBI) | 57534 |
RRID | AB_3671320 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q86YT6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053).
实验方案
Product Specific Protocols | |
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WB protocol for MIB1 antibody 83718-5-RR | Download protocol |
FC protocol for MIB1 antibody 83718-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |