• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83718-5-RR

    WB analysis using 83718-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83718-5-RR (MIB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83718-5-RR

    FC experiment of HeLa using 83718-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of SH-SY5Y using 83718-5-RR

    FC experiment of SH-SY5Y using 83718-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83718-5-RR

    Affinity and Kinetic Characterization of 83718-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83718-5-RR against Human MIB1 were performed. The affinity constant is 5.15 nM.

MIB1 Recombinant antibody

MIB1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83718-5-RR

产品说明书

CloneNo. 240676E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

DIP-1, DIP1, DIP 1, DAPK-interacting protein 1, DAPK interacting protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, DU 145 cells, HEK-293 cells, K-562 cells, C6 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83718-5-RR targets MIB1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MIB1 fusion protein Ag2507 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mindbomb homolog 1 (Drosophila)
别名 DIP-1, DIP1, DIP 1, DAPK-interacting protein 1, DAPK interacting protein 1
计算分子量 110 kDa
观测分子量 110 kDa
GenBank蛋白编号BC022403
基因名称 MIB1
Gene ID (NCBI) 57534
RRIDAB_3671320
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ86YT6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053).

实验方案

Product Specific Protocols
WB protocol for MIB1 antibody 83718-5-RRDownload protocol
FC protocol for MIB1 antibody 83718-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols