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MICA Monoclonal antibody, PBS Only

MICA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2C8

Cat no : 66384-1-PBS

Print datasheet

Synonyms

MIC A, MICA, PERB11.1

验证数据展示

  • WB analysis using 66384-1-Ig (same clone as 66384-1-PBS)

    WB analysis using 66384-1-Ig (same clone as 66384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66384-1-Ig (MICA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    IHC staining of human tonsillitis using 66384-1-Ig (same clone as 66384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66384-1-Ig (MICA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66384-1-PBS in a different storage buffer formulation.

产品信息

66384-1-PBS targets MICA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MICA fusion protein Ag3298 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name MHC class I polypeptide-related sequence A
Synonyms MIC A, MICA, PERB11.1
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight 43-50 kDa
GenBank Accession NumberBC016929
Gene Symbol MICA
Gene ID (NCBI) 100507436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ29983
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993, 11491531).