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验证数据展示

  • WB analysis using 83725-5-RR

    WB analysis using 83725-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83725-5-RR (MICA/MICB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83725-5-RR

    Affinity and Kinetic Characterization of 83725-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83725-5-RR against Human MICA were performed. The affinity constant is 1.55 nM.

MICA/MICB Recombinant antibody

MICA/MICB Recombinant Antibody for WB, ELISA
Cat No. 83725-5-RR

产品说明书

CloneNo. 240735D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

MICB, MICA, PERB11.1, MIC A, 240735D9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83725-5-RR targets MICA/MICB in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 MHC class I polypeptide-related sequence A
别名 MICB, MICA, PERB11.1, MIC A, 240735D9
计算分子量43 kDa
观测分子量50-60 kDa
GenBank蛋白编号AAH16929.1
基因名称 MICA
Gene ID (NCBI) 100507436
RRIDAB_3671325
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB.

实验方案

Product Specific Protocols
WB protocol for MICA/MICB antibody 83725-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols