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验证数据展示

  • WB analysis using 68297-1-Ig (same clone as 68297-1-PBS)

    WB analysis using 68297-1-Ig (same clone as 68297-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68297-1-Ig (same clone as 68297-1-PBS)

    IP experiment of HeLa using 68297-1-Ig (same clone as 68297-1-PBS)

    IP result of anti-MICAL1 (IP:68297-1-Ig, 4ug; Detection:68297-1-Ig 1:2000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68297-1-Ig (same clone as 68297-1-PBS)

    IF Staining of HeLa using 68297-1-Ig (same clone as 68297-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MICAL1 antibody (68297-1-Ig, Clone: 3F5D4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68297-1-Ig (same clone as 68297-1-PBS)

    FC experiment of HeLa using 68297-1-Ig (same clone as 68297-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MICAL1 (68297-1-Ig, Clone:3F5D4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 68297-1-Ig (same clone as 68297-1-PBS)

    WB analysis of RAW 264.7 using 68297-1-Ig (same clone as 68297-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 68297-1-Ig (same clone as 68297-1-PBS)

    WB analysis of HSC-T6 using 68297-1-Ig (same clone as 68297-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.

MICAL1 Monoclonal antibody, PBS Only

MICAL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 68297-1-PBS

产品说明书

CloneNo. 3F5D4

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

NICAL, NEDD9-interacting protein with calponin homology and LIM domains, MICAL, EC:1.14.13.225, 3F5D4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68297-1-PBS targets MICAL1 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 MICAL1 fusion protein Ag6578 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 microtubule associated monoxygenase, calponin and LIM domain containing 1
别名 NICAL, NEDD9-interacting protein with calponin homology and LIM domains, MICAL, EC:1.14.13.225, 3F5D4
计算分子量 118 kDa
观测分子量 120 kDa
GenBank蛋白编号BC052983
基因名称 MICAL1
Gene ID (NCBI) 64780
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TDZ2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.