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  • Featured Product
  • KD/KO Validated

MICAL2 Polyclonal antibody, PBS Only

MICAL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13965-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 13965-1-AP (same clone as 13965-1-PBS)

    WB analysis using 13965-1-AP (same clone as 13965-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 13965-1-AP (same clone as 13965-1-PBS)

    WB analysis of PC-3 using 13965-1-AP (same clone as 13965-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

    IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MICAL2 antibody included
  • IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

    IHC staining of human prostate cancer using 13965-1-AP (same clone as 13965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MICAL2 antibody included
  • IF Staining of HepG2 using 13965-1-AP (same clone as 13965-1-PBS)

    IF Staining of HepG2 using 13965-1-AP (same clone as 13965-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13965-1-AP (same clone as 13965-1-PBS)

    IF Staining of HepG2 using 13965-1-AP (same clone as 13965-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13965-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MICAL2 Ready-To-Use IHC Kit. MICAL2 primary antibody included.

产品信息

13965-1-PBS targets MICAL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MICAL2 fusion protein Ag4950 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microtubule associated monoxygenase, calponin and LIM domain containing 2
Synonyms
Calculated Molecular Weight 127 kDa
Observed Molecular Weight 95 kDa, 112 kDa
GenBank Accession NumberBC044577
Gene Symbol MICAL2
Gene ID (NCBI) 9645
RRIDAB_2143764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94851
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions. There are three known isoforms, MICAL1, MICAL2, and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, and a Lin11, Isl-1, and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variant of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.