验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells, BxPC-3 cells, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
18939-1-AP targets MIDN in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MIDN fusion protein Ag13492 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | midnolin |
| 别名 | Midnolin |
| 计算分子量 | 468 aa, 49 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC094778 |
| 基因名称 | MIDN |
| Gene ID (NCBI) | 90007 |
| RRID | AB_2878569 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q504T8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Midbrain nucleolar protein (midnolin, MIDN) is abundantly expressed in embryonic midbrain and localizes in the nucleus and nucleolus. MIDN mRNA was strongly and abundantly expressed during early developmental stages, and that the expression was not limited to midbrain but also expressed in diverse adult mouse tissues, including heart, spleen, lung, liver, skeletal muscle, kidney and testis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIDN antibody 18939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Biol CREB promotes beta cell gene expression by targeting Its coactivators to tissue-specific enhancers. | ||
Science The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation
| ||
Cancer Sci Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling |
