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MIF Recombinant antibody

MIF Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230529F2

Cat no : 83199-2-RR

Print datasheet

Synonyms

GIF, 230529F2

验证数据展示

  • WB analysis using 83199-2-RR

    WB analysis using 83199-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83199-2-RR (MIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 83199-2-RR

    IF Staining of NIH/3T3 using 83199-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MIF antibody (83199-2-RR, Clone: 230529F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of Neuro-2a using 83199-2-RR

    FC experiment of Neuro-2a using 83199-2-RR

    1x10^6 Neuro-2a cells were intracellularly stained with 0.25 ug MIF Recombinant antibody (83199-2-RR, Clone:230529F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83199-2-RR

    Affinity and Kinetic Characterization of 83199-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83199-2-RR against Mouse MIF were performed. The affinity constant is 0.131 nM.

经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, THP-1 cells, RAW 264.7 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNeuro-2a cells, RAW 264.7 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83199-2-RR targets MIF in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name macrophage migration inhibitory factor
Synonyms GIF, 230529F2
Calculated Molecular Weight13KD
Observed Molecular Weight13 kDa
GenBank Accession NumberNM_010798.2
Gene Symbol Mif
Gene ID (NCBI) 17319
RRIDAB_3670887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP34884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Macrophage migration inhibitory factor (MIF) is a homo-trimeric protein that acts as a pleiotropic pro-inflammatory cytokine. It is involved in various functions, including leukocyte recruitment, inflammation, immune responses, cell proliferation, tumorigenesis, and counter-regulation of glucocorticoids (GC). MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

实验方案

Product Specific Protocols
WB protocol for MIF antibody 83199-2-RRDownload protocol
IF protocol for MIF antibody 83199-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols