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MIF4GD Polyclonal antibody

MIF4GD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24288-1-AP

Print datasheet

Synonyms

SLIP1, MIFD, MIF4G, hSLIP1, AD023

验证数据展示

  • WB analysis using 24288-1-AP

    WB analysis using 24288-1-AP

    mouse testis tissue was subjected to SDS PAGE followed by western blot with 24288-1-AP (MIF4GD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24288-1-AP targets MIF4GD in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MIF4GD fusion protein Ag17931 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MIF4G domain containing
Synonyms SLIP1, MIFD, MIF4G, hSLIP1, AD023
Calculated Molecular Weight 256 aa, 29 kDa
Observed Molecular Weight~25 kDa
GenBank Accession NumberBC033759
Gene Symbol MIF4GD
Gene ID (NCBI) 57409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA9UHW6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIF4GD (MIF4G domain containing), also known as MIFD, AD023, or SLIP1, is a 222 amino acid protein that contains one MIF4G domain. Localized to both the nucleus and the cytoplasm, MIF4GD plays a role in the replication-dependent translation of histone mRNAs, which differ from most eukaryotic mRNAs in that they end with a stem-loop instead of a poly-A tail. Specifically, MIF4GD interacts with SLBP, eIF4G and DAP-5. Via its interaction with SLBP, MIF4GD is thought to be tethered to the stem loops of histone mRNAs where it may facilitate the circularizing of the mRNAs, thereby enhancing their translation. Depletion of MIF4GD results in reduced histone translation and may lead to cell death, suggesting that MIF4GD plays an important role in cell survival. Two isoforms of MIF4GD exist due to alternative splicing events.

实验方案

Product Specific Protocols
WB protocol for MIF4GD antibody 24288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols