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验证数据展示

  • WB analysis using 83475-2-RR

    WB analysis using 83475-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83475-2-RR (MIG6; ERRFI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83475-2-RR

    FC experiment of HepG2 using 83475-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MIG6; ERRFI1 Recombinant antibody (83475-2-RR, Clone:240447C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83475-2-RR

    Affinity and Kinetic Characterization of 83475-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83475-2-RR against Human MIG6; ERRFI1 were performed. The affinity constant is 2.04 nM.

View 3D epitope

MIG6; ERRFI1 Recombinant antibody

MIG6; ERRFI1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83475-2-RR

产品说明书

CloneNo. 240447C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ERRFI1, 240447C3, MIG-6, MIG6, MIG 6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2480/ 100 μL

浓度: 1000 ug/ml

库存：现货

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经过测试的应用

Positive WB detected inHCT 116 cells, HepG2
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83475-2-RR targets MIG6; ERRFI1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MIG6; ERRFI1 fusion protein Ag33341 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ERBB receptor feedback inhibitor 1
别名 ERRFI1, 240447C3, MIG-6, MIG6, MIG 6
计算分子量51kd
观测分子量 51 kDa
GenBank蛋白编号NM_018948
基因名称 MIG6
Gene ID (NCBI) 54206
RRIDAB_3671103
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UJM3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.

实验方案

Product Specific Protocols
WB protocol for MIG6; ERRFI1 antibody 83475-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols