验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HepG2 |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83475-2-RR targets MIG6; ERRFI1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | MIG6; ERRFI1 fusion protein Ag33341 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ERBB receptor feedback inhibitor 1 |
| 别名 | ERRFI1, 240447C3, MIG-6, MIG6, MIG 6 |
| 计算分子量 | 51kd |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | NM_018948 |
| 基因名称 | MIG6 |
| Gene ID (NCBI) | 54206 |
| RRID | AB_3671103 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UJM3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIG6; ERRFI1 antibody 83475-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |